mouse serum total ige  (R&D Systems)

Journal: Redox Biology

Article Title: Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis

doi: 10.1016/j.redox.2026.104059

Figure Lengend Snippet: Impact of l -lactate metabolism on HDM-induced murine airway inflammation and Th2 immune response. (A) LDH enzyme activity detection in serum (left) and lung tissue homogenates (right) of mice in each group. (B) Quantitative analysis of lactate concentrations in serum and lung tissue. (C) Diff-Quik staining images of BALF cell smears (left, red box indicates magnified field) and statistics of eosinophil proportions (right). (D) H&E staining (top, showing inflammatory infiltration) and PAS staining (bottom, showing goblet cells and mucus) of lung tissue sections. Red arrows indicate lesion areas. Right side shows pathological scores. (E) ELISA detection of IL-4, IL-5, and IL-13 concentrations in BALF. (F) ELISA detection of serum total IgE and HDM-specific IgE levels. (G) Flow cytometric analysis of Th1 (IFN-γ + CD4 + ) and Th2 (IL-4 + CD4 + ) cell subsets in mediastinal lymph nodes (mLN) and spleen. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant (One-way ANOVA).

Article Snippet: Mouse serum total IgE and HDM-specific IgE (R&D Systems), as well as IL-4 (E-EL-M0043), IL-5 (E-EL-M0722), IL-13 (E-EL-M0727), HMGB1 (E-EL-M0676/E-EL-H1554), IL-1β (E-EL-H0149), and IL-18 (E-EL-H0253) in BALF or cell supernatants were all detected using ELISA kits from Elabscience.

Techniques: Activity Assay, Diff-Quik, Staining, Enzyme-linked Immunosorbent Assay

Journal: Redox Biology

Article Title: Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis

doi: 10.1016/j.redox.2026.104059

Figure Lengend Snippet: Effect of blocking ATP6V1B2 lactylation on mouse asthma phenotypes and in vivo pyroptosis signals. (A) H&E and PAS staining and scoring of AAV-transduced mouse lung tissues. (B) Flow cytometric analysis of eosinophils (CD11C − CD45.2 + Siglec-F + ) in BALF. (C) Detection of serum total IgE and HDM-specific IgE levels. (D) ELISA analysis of Th2 cytokines (IL-4, IL-5, IL-13) in BALF. (E) Measurement of lactate levels in serum and lung tissue homogenates. (F) Immunofluorescence staining of in situ Pan-Kla (green) and LAMP1 (red) in lung tissue frozen sections. Scale bar: 100 μm. (G) Immunoblot analysis of GSDME and Caspase-3 cleavage in lung tissue. (H) Lung tissue LDH activity detection. Data are expressed as Mean ± SEM. n = 10 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Mouse serum total IgE and HDM-specific IgE (R&D Systems), as well as IL-4 (E-EL-M0043), IL-5 (E-EL-M0722), IL-13 (E-EL-M0727), HMGB1 (E-EL-M0676/E-EL-H1554), IL-1β (E-EL-H0149), and IL-18 (E-EL-H0253) in BALF or cell supernatants were all detected using ELISA kits from Elabscience.

Techniques: Blocking Assay, In Vivo, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, In Situ, Western Blot, Activity Assay

Journal: Microorganisms

Article Title: Wild-Mouse-Derived Gut Microbiome Transplantation in Laboratory Mice Partly Alleviates House-Dust-Mite-Induced Allergic Airway Inflammation

doi: 10.3390/microorganisms12122499

Figure Lengend Snippet: Compared with SPF-microbiome-associated mice, wild-mouse microbiome-associated mice showed reduced allergic airway inflammation: ( A ) Experimental design showing the timeline of antibiotic treatment (ABX) and microbiome transplantation (FMT) as well as house dust mite (HDM) sensitization and challenges before euthanization of male and female wild mice (wild) and specific pathogen-free (SPF) microbiome-associated BALB/c mice; ( B ) body weight; ( C ) bronchoalveolar fluid (BAL) cell counts; ( D ) numbers of eosinophils (CD11c-CD3-CD19-CD11b+Ly6G-SigF+) in the BAL fluid; ( E ) numbers of neutrophils (CD11c-CD3-CD19-CD11b+Ly6G+) in the BAL fluid; ( F ) numbers of T cells (CD11c-CD3+MHCII-) in the BAL fluid; ( G ) numbers of B cells (CD11c-CD19+MHCII+) in the BAL fluid; ( H ) lung concentration of TNF-α; ( I ) serum concentration of TNF-α; ( J ) serum total IgE concentrations; ( K ) histopathological inflammatory score in lung tissue, including representative images of H&E-stained sections ( L , M ); ( N ) percentage of mucin-positive bronchi, including representative images of PAS-stained sections ( O , P ), are shown for the wild ( n = 13) and SPF ( n = 8) microbiome-associated mice when euthanized at 7 weeks of age. All p values are given (significance < 0.05). The mean and SEM are shown.

Article Snippet: Serum total IgE concentrations were measured at euthanasia using a Mouse IgE ELISA Kit (Bethyl Laboratories, Montgomery, TX, USA) as previously described [ ].

Techniques: Transplantation Assay, Concentration Assay, Staining

Journal: Allergy

Article Title: Mast cells and sphingosine-1-phosphate underlie prelesional remodeling in a mouse model of eczema

doi: 10.1111/all.13310

Figure Lengend Snippet: (a) Alpha chain for the high-affinity receptor for IgE mRNA levels in OVA-treated samples (grey bars), normalized to saline (empty bars) treated samples and GAPDH, n = 4 saline animals, 6 OVA animals with duplicate determinations (*** p = 0.0002). (b, c) Methylene blue-stained skin sections after saline (b) or OVA (c) treatment. Arrows and inserts show examples of intact (green) or degranulated (red) mast cells (MC). Panels b-c are representative images. Bar = 50 μm. (d) Percent MC degranulation determinations in saline- and OVA-treated samples (* p = 0.0352, fifty 40x- total images/310–485 total MC; 4 skin sections/2 mice/treatment group). (e) Determinations of total serum IgE after one saline or OVA treatment (1 Patch), n = 6 animals/treatment group, averages of duplicate determinations and after three saline or OVA treatments (3 Patches), n = 9 animals/treatment group, averages of duplicate determinations (*** p = 0.0001). (f) Sphingosine-1-phosphate (S1P) content of mouse skins after saline or OVA treatment, n = 8 saline and 5 OVA samples. ** p = 0.0088 (g) Sphingosine kinase (SphK)1 mRNA levels in OVA-treated samples (n = 4), normalized to saline (n = 3) with duplicate measurements (* p = 0.0152). Statistical differences were determined with unpaired 2-tailed Student’s t test with Welch’s correction.

Article Snippet: Mouse total serum IgE was measured by ELISA (R&D Systems, Minneapolis, MN).

Techniques: Saline, Staining

Journal: PLoS ONE

Article Title: Persistence of Asthmatic Response after Ammonium Persulfate-Induced Occupational Asthma in Mice

doi: 10.1371/journal.pone.0109000

Figure Lengend Snippet: Total serum IgE, IgG1 and IgG2a were measured using a standard ELISA. Experimental groups are as in . A ) Mean ± SEM of total serum IgE. B ) Mean ± SEM of total serum IgG1. C ) Mean ± SEM of total serum IgG2a. *p<0.05, **p<0.01 compared with DMSO/SAL, +p<0.05 compared with DMSO/PA. No significant differences were found in the other groups studied at different time intervals. AP, ammonium persulfate; DMSO, dimethylsulfoxide; SAL, saline.

Article Snippet: The Mouse ELISA kits (Bethyl Laboratories, Inc., Montgomery, USA) were used to measure total serum IgE, IgG1 and IgG2a (diluted samples 1/5, 1/12500 and 1/5000, respectively).

Techniques: Enzyme-linked Immunosorbent Assay